Journal: International Journal of Molecular Sciences
Article Title: Effective Delivery of Arsenic Trioxide to HPV-Positive Cervical Cancer Cells Using Optimised Liposomes: A Size and Charge Study
doi: 10.3390/ijms19041081
Figure Lengend Snippet: Arsenic uptake and toxic response in the control cells and HeLa cells. ( a ) A comparative study of arsenic concentration per cell in CRL-1790, human epidermal keratinocyte (HK), and HeLa—as determined via ICP-MS after 24 h of treatment with media, control liposomes, ATO lipo, and free ATO; ( b ) Cell survival following the exposure of cervical cancer and control cell lines to ATO lipo for 24 h—assessed using the MTT assay; ( c ) Toxicity–uptake ratio of ATO lipo and free ATO in HeLa cells compared to control cell lines, after treatment for 24 h. ATO lipo is effective in generating toxicity in HeLa cells, while having a non-toxic effect on the normal cells; ( d ) The IC 50 values for three cell lines following 24 h of ATO liposomal treatment. Data are displayed as mean ± SD from three replicates; * p < 0.05, ** p < 0.01.
Article Snippet: Two cervical cancer cell lines, HeLa and HT-3 (ATCC, Manassas, VA, USA), and two control cell lines, human epidermal keratinocytes HK (Fisher Scientific, Loughborough, UK) and human colon epithelial cells CRL-1790 (ATCC, Manassas, VA, USA), were employed in this study.
Techniques: Concentration Assay, MTT Assay